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WebNov 26, 2014 · Subsequently the ΔCT for each sample was determined using the equation ΔCT = CTtarget gene – CThousekeeping gene. ΔCT = CT (IL-2) – CT (GAPDH) ΔCT = CT (SOCS-1) – CT (GAPDH). Statistical analysis. The results are expressed as means ± standard errors (SE) and medians + ranges. Differences in variables between the two … WebActin YFG delta Ct (CtGOI - Cthousekeeping genes) delta delta Ct ( delta Ct- Avg.CtControl) Control 7 12 5 0 Treated 11 16 …View the full answer. Transcribed image text: Question 17 Not yet answered Calculate the deltadelta-Ct value for the following Ct values from a qPCR experiment: Gene Control Samples Treatment Samples YFG 12 16 … WebJan 1, 2024 · The housekeeping genes for normalizing ERβ and PR expression included β-actin (Rn00667869_m1). The determination of the gene transcript levels in each sample was obtained by the ΔΔCT method. For each sample, the threshold cycle (Ct) was determined and normalized to the average of the housekeeping gene (ΔCt = CtUnknown - … opticians boots oxford street