WebApr 7, 2024 · It might be related to the F-actin disruption caused by ES, since the distribution of GLUT1 on cell surface could be changed from clusters to dispersed states when the actin cytoskeleton depolymerized by cytochalasin B. We further examined the glucose uptake capacity of cells after the ES using fluorescence glucalogue (2-NBDG), which has the ... WebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 …
Effect of cytochalasin B on glucose uptake, utilization, …
Web2.2 Assaying Insulin-Stimulated Glucose Uptake 1. Serum-free media: DMEM (with 4.5 g/L glucose, l -glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin (BSA). 2. 2-Deoxyglucose (Sigma) stock: 100 m M (16.4 mg/mL in double-distilled water). Store at 2°–4°C up to 2 wk. 3. WebNov 11, 2008 · Non-carrier-mediated uptake was determined in parallel wells containing 10 μmol/L cytochalasin B. Protein concentration was measured by the modified Bradford protein assay. Cell-associated radioactivity was determined by scintillation counting. The mean counts/min were expressed as pmol/min/mg protein from determinations done in … the outsiders hbo
Cytochalasin B CAS 14930-96-2 SCBT - Santa Cruz …
WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … WebAbstract. Cytochalasin B (17-3 microM) virtually abolished 3-O-methyl-D- [U-14C]glucose uptake and D- [5-3H]glucose utilization in tumoral insulin-producing cells of the RINm5F … WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ... the outsiders hbo 2020