Diethyl pyrocarbonate-treated water
WebDiethyl-pyrocarbonate (DEPC)-treated water is used to dissolve RNA and 2-amino-2-hydroxymethyl-1,3-propandiol (Tris) is frequently added to the RNA dissolving solution in order to stabilize the RNA. In the present study, RNA was isolated from mouse liver, and then the influence of DEPC-treated water and Tris-buffer on RNA measurement was ...
Diethyl pyrocarbonate-treated water
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WebJun 22, 2016 · Each bee TRIzolReagent, guanidineisothiocyanate RNAextraction (Invitrogen, Carlsbad, CA), totalRNA extractedfollowing manufacturer’sstandard protocol. After isopropanol precipitation, RNApellet diethyl-pyrocarbonate (DEPC)-treated water Ribonu-clease Inhibitor (Invitrogen, Carlsbad, CA) 808C molecularanalyses. 2.6. WebIt is supplied at a concentration of 5 mg/mL in diethyl pyrocarbonate-treated distilled water. Glycogen will not interfere with 260/280 readings. This glycogen product is isolated from mussel, a biological source, as are most other preparations of this coprecipitant.
WebChemtrac Inc. is a leading manufacturer of process monitoring and control instruments used in water, wastewater, and other industrial processes. Our reputation and growth has … WebRinse tanks with DEPC (diethyl pyrocarbonate)-treated and autoclaved water before use (see below). Glassware and plasticware Treat glassware and plasticware with RNase-inactivating agents. Glassware should be baked at +180°C for at least 4 hours. Note, however, that autoclaving alone is not sufficient to eliminate RNases from your experiments.
WebSep 20, 2024 · DEPC treated water, if not prepared very accurately (you have to make sure the all the diethyl pyrocarbonate has been removed!), will risk inhibiting the transcription reaction by also... WebWater destined for molecular biology include nuclease-free water and diethyl pyrocarbonate (DEPC)-treated water, which ensure DNA and RNA will not be degraded by the presence of enzymes. HPLC and LC-MS grade water are recommended for analytical chromatography. Others, such as ATP-free water, are ideal for biochemical assays.
WebDiethyl pyrocarbonate (DEPC), a powerful acylating agent, has several functions in Molecular Biology and Biochemistry. An acylating agent is one in which an acyl (RCO-) radical group is introduced into molecules of a …
WebThe necessity for diethyl pyrocarbonate (DEPC)-treated solutions in RNA molecular biological procedures was tested by preparing RNA from a variety of tissues and tissue-cultured cells using either DEPC-treated, autoclaved solutions or pure Milli-Q PF water dispensed directly from the system. merlins sports bar great yarmouthWebWater, DEPC-Treated, Molecular Biology Grade Sterile-filtered water treated with diethyl pyrocarbonate (DEPC).; CAS Number: 7732-18-5; Synonyms: Water; Linear Formula: … merlin stafford wax jacketWebOct 10, 2012 · If you have ever worked with RNA, you know about DEPC (diethylpyrocarbonate). You add it to water at a concentration of 0.1%, shake or stir, incubate at 37 ° C for two hours or at room temperature overnight and, as if targeted by a magic bullet, the RNAses that may have been in the water are gone. merlin staff lyricsWebDiethylpyrocarbonate water C6H12O6 CID 87579661 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological ... how quickly can alt levels changeWebDiethyl pyrocarbonate (DEPC)-treated H2O. Distilled H2O. Diethyl pyrocarbonate (DEPC) Add 1 mL of fresh DEPC to 1 L of H2O. Shake well to disperse the DEPC through the … merlin stabilizer screw replacementWebUltraPure™ DEPC-treated Water is suitable for use with RNA. It is prepared by incubating with 0.1% diethylpyrocarbonate (DEPC), and is then … merlins sheffieldWebProduct Name Diethyl pyrocarbonate Cat No. : AC170250000; AC170250025; AC170250050; AC170250250; AC170251000; AC170255000 CAS No 1609-47-8 … how quickly can a horse founder on grass