Engineered gfp as a vital reporter in plants
WebChiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J. Engineered GFP as a vital reporter in plants. Curr Biol. 1996 Mar 1; 6 (3):325–330. [Google Scholar] Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY. Understanding, improving and using green fluorescent proteins. Trends Biochem Sci. 1995 Nov; 20 (11):448–455. WebApr 12, 2024 · An accurate visual reporter system to assess homology-directed repair (HDR) is a key prerequisite for evaluating the efficiency of Cas9-mediated precise gene editing. Herein, we tested the utility of the widespread promoterless EGFP reporter to assess the efficiency of CRISPR/Cas9-mediated homologous recombination by …
Engineered gfp as a vital reporter in plants
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WebEngineered GFP as a vital reporter in plants Background: The green fluorescent protein (GFP) of the jellyfish Aequorea victoria has recently been used as a universal reporter in … WebMay 14, 2009 · To obtain a genetic marker to observe and study the interaction of Sclerotinia sclerotiorum with its hosts, isolates ND30 and ND21 were transformed using pCT74 and gGFP constructs, both containing genes for the green fluorescent protein (GFP) and hygromycin B phosphotransferase.
WebWe show that codon-modified forms of gfp, produced for use in plants, function effectively in Aspergillus nidulans both as gene expression reporters and as vital reporters for … WebGreen fluorescent protein (GFP) has emerged as a powerful new tool in a variety of organisms. An engineered sGFP(S65T) sequence containing optimized codons of highly expressed eukaryotic proteins has provided up to 100-fold brighter fluorescence signals than the original jellyfish GFP sequence in plant and mammalian cells.
WebTL;DR: It is reported that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants, and the codon usage effect might be universal, allowing the design of recombinant proteins with high expression efficiency in evolutionarily distant species such as humans and maize. WebHere, we report that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants. Results We show that a re …
WebEngineered GFP gives faster and brighter fluorescent signals. Maize mesophyll protoplast were electroporated with three plasmids expressing (a,d) the jellyfish GFP (GFP), (b,e) synthetic GFP (sGFP) or (c,f) a mutant S65T sGFP (sGFP (S65T)). The transfected protoplast were observed after (a-c) 4 h or (d-f) 1 6 h of incubation.
WebApr 1, 2001 · GFP fusions have also been used to study both the nuclear export pathway in plants ( Haasen et al ., 1999) and trafficking of protein through plasmodesmata ( Imlau … headlight restoration companyWebFeb 29, 1996 · Here, we report that an extensively modified GFP is a versatile and sensitive reporter in a variety of living plant cells and in transgenic plants. Results We show that a … headlight restoration cost ukWebMar 1, 1996 · The green-fluorescent protein (GFP) of the jellyfish Aequorea victoria has recently been used as a universal reporter in a broad range of heterologous living cells … gold pass californiaWebJul 20, 2009 · Green fluorescent protein (GFP) as gene expression reporter and vital marker for studying development and microbe-plant interaction in the tobacco pathogen Phytophthora parasitica var. nicotianae. FEMS Microbiology Letters176, … headlight restoration deetWebBy fusing the genes encoding green fluorescent protein (GFP) and β-glucuronidase (GUS) we have created a set of bifunctional reporter constructs which Fusions between green … gold pass bring a friend discount cedar pointWebMar 4, 2024 · Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In the first report of prime … headlight restoration descriptionWebEngineered GFP as a vital reporter in plants Previous ArticleCodon usage limitation in the expression of HIV-1 envelope glycoprotein Next ArticleThe nascent polypeptide … headlight restoration denver