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Hif1α antibody

WebWestern blot analysis of HIF-1α on lysate from HeLa stimulated with CoCl2, cobalt chloride. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α antibody. Show More Show Less WebBiological Strategies: Western Blot: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. Simple Western: HIF-1 alpha Antibody [NB100-449] - …

Purified Mouse Anti-Human HIF-1α - BD Biosciences

WebMonoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts … Web6 de mai. de 2013 · GST-p300 CH1 protein (∼10 µg GST only as a negative control) was mixed with His-HIF1α FL, 15 µl of glutathione sepharose 4 Fast Flow beads, and novobiocin (100–400 µM). Bound proteins were then detected by Western blotting with anti-HIF1α antibody. Quantified HIF1α is depicted in the graph (lower panel). shashi tharoor on agneepath scheme https://aminokou.com

HIF1A Antibody (D5F3M) Cell Signaling Technology

WebHIF1β is an aryl hydrocarbon receptor nuclear translocator (Arnt). Under normoxic conditions, HIF1α undergoes prolyl hydroxylation, which leads to degradation of HIF1α … WebAnti-HIF-1α Antibody is a Rabbit Polyclonal Antibody for detection of HIF-1 alpha also known as ARNT interacting protein or HIF-1 alpha & has been validated in WB. - Find MSDS or SDS, a COA, data sheets and more information. WebASAP3 overexpression counteracts the inhibition of lung adenocarcinoma progression caused by HIF-1α knockdown both in vitro and in vivo. Conclusion: Our results identify ASAP3 as a downstream target of HIF-1α that is critical for metastatic progression in lung adenocarcinoma. Keywords: lung adenocarcinoma, ASAP-3, hypoxia-inducible factor-1α ... shashi tharoor in un

HIF1A Gene - GeneCards HIF1A Protein HIF1A Antibody

Category:Human/Mouse/Rat HIF-1 alpha/HIF1A Antibody MAB1536: R&D …

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Hif1α antibody

ASAP3 is a downstream target of HIF-1α and is critical for progr

Web18 de mar. de 2024 · Membranes were blocked with 5% milk powder in 1× PBS-T for 1 h and then incubated overnight at 4 °C with a mouse anti-HIF-1α monoclonal antibody in … Web4 de mar. de 2024 · As shown in Fig. 5B, anti-HIF-1α antibody immunoprecipitation successfully pulled down HIF-1α from the lysates of both MDA-MB-231 (lane 1) and HeLa (lane 2) cells.

Hif1α antibody

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WebPolyclonal Antibody for studying HIF1A. Cited in 194 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, HIF-1α Antibody (CST #3716) is ready to ship. Web9 de abr. de 2024 · Nanomaterials are gaining increasing attention as innovative materials in medicine. Among nanomaterials, zinc oxide (ZnO) nanostructures are particularly appealing because of their opto-electrical, antimicrobial, and photochemical properties. Although ZnO is recognized as a safe material and the Zn ion (Zn2+) concentration is strictly regulated …

WebProduct Specific Information. MA1-516 detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha. MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry ... WebAntibodies that detect HIF1A can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. …

WebA specific band was detected for HIF-1 alpha/HIF1A at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat HIF-1 alpha/HIF1A Monoclonal Antibody (Catalog # MAB1536). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Knockout Validated View Larger. Web11 de abr. de 2024 · The following antibodies were used: p53, PHD2, MMP2, HIF-1α, MRP1, and GAPDH, and the secondary antibody (detailed in Supplementary Table S1). Quantitative RT-PCR: In order to further compare the mRNA transcription levels of 3d-ACS and 2d cultured GC cells, the total RNA in the released cells was extracted using Trizol …

Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of human origin. Anti-HIF1a Antibody (H1alpha 67) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC (P); also reactive with additional …

Web9 de jun. de 2024 · Chromatin was diluted and input chromatin was collected. Remaining chromatin was used for ChIP by adding 4 μg mouse anti–HIF-1α (Abcam; ab1) as the antibody of interest, 4 μg mouse anti-histone H3 (Abcam, ab1220) as a positive control, and mouse anti-GFP antibody (Abcam, ab1218) as a negative control. Antibodies were … shashi tharoor mother tongueWeb21 de mar. de 2024 · Rockland Anti-HIF1 alpha (MOUSE) Monoclonal Antibody - 200-301-F45 and Anti-Hif-1α hydroxy P564 (RABBIT) Antibody - 100-401-A25; Abcam antibodies for HIF1A. HIF-1 alpha; Buy Protein products for research. RayBiotech Recombinant Human HIF-1-alpha, HEK293 Transfected Cell Culture Supernatant and Recombinant Human … porsche club western australiaWebTranswell chamber was purchased from Corning (NY, USA). The antibodies to HIF-1α ( GTX127309) were purchased from GeneTex (TX, USA). The antibodies to Phospho- JNK1/2 /3 ( AP0631) and JNK1 / JNK3 ( A5051) (3) Neuroinvasion of SARS-CoV-2 in human and mouse brain bioRxiv September 27, 2024 Eric Song et al. porsche cockpitWebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … shashi tharoor speechesWeb21 de mar. de 2024 · HIF1A (Hypoxia Inducible Factor 1 Subunit Alpha) is a Protein Coding gene. Diseases associated with HIF1A include Retinal Ischemia and Enchondromatosis, … porsche coburgWebKnockout Validated Rabbit Polyclonal anti-HIF-1 alpha Antibody - BSA Free. Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, Dual ISH-IHC + more. Reactivity: … shashi tharoor emailWeb8 de abr. de 2024 · The percentage of positive tumour cells was scored for each antibody, using only nuclear staining for HIF-1α, and only membranous staining for CA-IX. Cut-off values of the percentages for dichotomisation of the data were in accordance with the cut-off values used in previous studies performed in our institution [ 13 , 14 ], and were 0.5% for … shashi tharoor genre