WebCell lines and cell culture. The human normal pancreatic ductal epithelial cell line (HPDE6-C7) was purchased from Genio Biotechnology Co., Ltd. The human PDAC cell lines AsPC-1, BxPC-3, CFPAC-1, PANC-1, and SW1990 were purchased from Genechem Co., Ltd. ... In AsPC-1 and PANC-1 cells, miR-140-5p mimics significantly reduced the relative ... WebThe human PDAC cell lines PANC-1 and MIA-PACA-II cell were cultured in DMEM (#11965084, Gibco) supplemented with 10% fetal bovine serum (FBS) along with 100 U/ml penicillin-streptomycin (#10378016, Gibco) under 37°C and 5% CO 2 conditions. PANC05.04 cells were cultured in F12k media (#21127022, Gibco) supplemented with …
A novel pancreatic tumour and stellate cell 3D co-culture spheroid ...
WebJan 8, 2024 · (A) PANC-1 cells were transfected with Poly (I:C)-HMW, Poly (I:C)-LMW, and Poly (dA:dT) for 9 h. Cells were then lysed and subjected to western blotting to detect the expression of pPKR, PKR, TLR3, RIG-I, MDA5, LGP2, and DHX29, with β-actin as the loading control. Data are representative of three independent experiments. WebJul 10, 2024 · Each line has been characterized for clonality; confirmation of endogenous KRAS gene removal; identification of transgene insertion site (s); growth characteristics … excavator buckets for sale ebay
Cellosaurus cell line PANC-1 (CVCL_0480)
WebPancreatic cancer (PANC) is a dangerous type of cancer that is a major cause of mortality worldwide and exhibits a remarkably poor prognosis. To date, discovering anti-PANC … WebCell line: PANC-1 : DSMZ no.: ACC 783: Species validation: human (Homo sapiens)Cell type: pancreas carcinoma : Origin: established in 1972 from the ductal cells of a tumor … WebMaintenance of PANC-1 cells before spheroid generation. After thawing from liquid nitrogen, PANC-1 cells were maintained in Nunclon Delta T25 cell culture flasks in Gibco DMEM medium supplemented with 10% Gibco FBS and 1% Pen-Strep for 2 passages before seeding for spheroid generation. ATCC protocol was followed for subculturing. bryan welsh nmma