WebPBS-Triton Wash Buffer - For wash sequences immediately after permeabilization, before blocking, and after treatment with antibodies (before staining with nuclear dyes), use PBS containing 0.05 percent Triton X-100. PBS-Triton Wash Buffer with Blocking Serum - For wash sequences between the primary and secondary antibody incubations and ... WebPBS-Triton Wash Buffer with Blocking Serum- For wash sequences between the primary and secondary antibody incubations and immediately after the secondary antibody treatment, use PBS with 0.05 percent Triton X-100 and 1 percent normal host (goat) serum.
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WebDetergent-based cell lysis. Both denaturing and non-denaturing cell lysis reagents may be used for protein extraction procedures. Denaturing detergents such as SDS bind to both membrane (hydrophobic) and non-membrane (water-soluble, hydrophilic) proteins at concentrations below the CMC (i.e., as monomers). The reaction is equilibrium driven ... WebRemove the PBS-Triton Wash Buffer and block nonspecific secondary antibody binding sites with 10-percent normal host serum Blocking Buffer. Treat the adherent cells for 60 minutes at room temperature with the Blocking Buffer and slowly rotate the Petri dishes as the cells are being blocked on an orbital shaker at 5-10 revolutions per minute. surv of broadway